Laboratory Notes for BIO 1003

© 30 August 1999, John H. Wahlert & Mary Jean Holland

ENERGY PRODUCTION BY CATABOLISM

Materials

• 3 jars, each with rubber stopper with two holes;
  jar 1 contains live sprouted peas
  jar 2 contains sprouted, boiled peas
  jar 3 contains dry peas
  thistle tube inserted in one stopper hole
  U-shaped exhaust tube in other hole
  each U-tube exits in phenol red solution in an adjacent graduated cylinder
  beaker of distilled water to pour into thistle tubes
• phenol red solution in graduated cylinder
  pipette for instructor to blow into

• 1 incubator set at 37 degrees C

• 18 fermentation tubes (3 at each of 6 stations)
• 18  40 ml beakers (3 at each of 6 stations)
• 6  100 ml beakers (1 at each of 6 stations)

• 1   400 ml beaker of brewer's yeast in water suspension
• 2   250 ml stock bottles of 1% starch solution
• 2   250 ml stock stock bottles of 10% glucose solution
• 2   250 ml stock stock bottles of distilled water

• 6   15 cm rulers

Themes

• Organisms get energy from food.
• Catabolism, the breakdown of food molecules, such as glucose, ultimately yields the energy carrying molecules ATP and carbon dioxide gas as a waste product

Web Resources

• Fermentations Important to Humans, ©2000 Timothy Paustian, University of Wisconsin-Madison

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