Laboratory Notes for BIO 1003

© 30 August 1999, John H. Wahlert & Mary Jean Holland


Enzymes

Materials

Equipment:
  • 3 water baths (40, 60, and 80 degrees C) with metal test tube racks
  • 6 hot plates (with 400 ml beaker)
Glassware:
  • 8   400 ml beakers with tap water
    1 in each ice bucket near 0 degrees C (2 beakers)
    1 on each hotplate with tap water and boiling chips (6 beakers)
  • 6   250 ml beakers with tap water at room temperature
  • 126 disposable test tubes (13 by 100 mm, holds about 8 ml), 21 per group
Solutions:
  • potato extract (catecholase source): 4   60 ml brown bottles; 2 in each ice bucket
  • catechol (substrate): 4   60 ml brown bottles; 2 in each ice bucket
  • hydroquinone (substrate mimic): 4   60 ml brown bottles; 2 in each ice bucket
  • 6 pH 2 buffer solution in 30 ml Wheaton dropper bottle
  • 6 pH 4 buffer solution in 30 ml Wheaton dropper bottle
  • 6 pH 6 buffer solution in 30 ml Wheaton dropper bottle
  • 6 pH 7 buffer solution in 30 ml Wheaton dropper bottle
  • 6 pH 8 buffer solution in 30 ml Wheaton dropper bottle
  • 6 pH 10 buffer solution in 30 ml Wheaton dropper bottle
  • 6 pH 12 buffer solution in 30 ml Wheaton dropper bottle
Other:
  • 3 metal test tube racks, 1 for each water bath
  • 2 ice buckets with ice chips
  • boiling chips for each beaker on hot plate
  • 2 small bottles of PTU crystals
  • metal scoop for PTU crystals

Themes

  • Catalysts speed up a chemical reaction and are not changed by it.
  • Enzymes are proteins made by cells that function as catalysts.
  • Enzymes are specific as to what reactants are accepted into the active site.
  • Enzymes work effectively under specific physiological conditions: temperature, pH, and solutes present.

Return to Enzymes lab or Index.

Last updated 14 June 2006 (JHW)