Laboratory Notes for BIO 1003
© 30 August 1999, John H. Wahlert & Mary Jean Holland
Enzymes
Materials
Equipment:
- 3 water baths (40, 60, and 80 degrees C) with metal test tube racks
- 6 hot plates (with 400 ml beaker)
Glassware:
- 8 400 ml beakers with tap water
1 in each ice bucket near 0 degrees C (2 beakers)
1 on each hotplate with tap water and boiling chips (6 beakers)
- 6 250 ml beakers with tap water at room temperature
- 126 disposable test tubes (13 by 100 mm, holds about 8 ml), 21 per group
Solutions:
- potato extract (catecholase source): 4 60 ml brown bottles; 2 in each ice bucket
- catechol (substrate): 4 60 ml brown bottles; 2 in each ice bucket
- hydroquinone (substrate mimic): 4 60 ml brown bottles; 2 in each ice bucket
- 6 pH 2 buffer solution in 30 ml Wheaton dropper bottle
- 6 pH 4 buffer solution in 30 ml Wheaton dropper bottle
- 6 pH 6 buffer solution in 30 ml Wheaton dropper bottle
- 6 pH 7 buffer solution in 30 ml Wheaton dropper bottle
- 6 pH 8 buffer solution in 30 ml Wheaton dropper bottle
- 6 pH 10 buffer solution in 30 ml Wheaton dropper bottle
- 6 pH 12 buffer solution in 30 ml Wheaton dropper bottle
Other:
- 3 metal test tube racks, 1 for each water bath
- 2 ice buckets with ice chips
- boiling chips for each beaker on hot plate
- 2 small bottles of PTU crystals
- metal scoop for PTU crystals
Themes
- Catalysts speed up a chemical reaction and are not changed by it.
- Enzymes are proteins made by cells that function as catalysts.
- Enzymes are specific as to what reactants are accepted into the active site.
- Enzymes work effectively under specific physiological conditions: temperature, pH, and solutes present.
Return to Enzymes lab or Index.
Last updated 14 June 2006 (JHW)
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