Laboratory Notes for BIO 1003
© 30 August 1999, John H. Wahlert & Mary Jean Holland
USE OF THE MICROSCOPE
- Compound microscopes with 3 objectives (1 per student)
Solutions (6 sets):
- microscope slides
- cover slips
- Lugol's iodine (30 ml Wheaton dropper bottle)
- methylene blue (30 ml Wheaton dropper bottle)
- isotonic saline solution for cheek and onion cells (30 ml Wheaton dropper bottle)
- transparent 4 cm ruler pieces
- sterile sticks for collecting cheek cells (24)
- 6 scalpels for cutting and peeling onion skin
- lens tissue
- plastic droppers
- Proper handling of microscopes to avoid damage.
- Strategies for optimizing observation.
- Staining to make structures visible and differentiate cell from background.
- Visible structures in eukaryotic cells.
- Estimating the dimensions of microscopic objects.
- Cell theory: Matthias Schleiden (plants: 1838) and Theodor Schwann (animals: 1839): all living things are made up of cells.
- Microscope skills will be needed in many future lab exercises.
Return to Microscope lab or Index.
Last updated 7 June 2006 (JHW)